GSM1958347: Linear plasmid; Synthetic plasmid; RNA-Seq - SRA

SRX1456272: GSM1958347: Linear plasmid; Synthetic plasmid; RNA-Seq
1 ION_TORRENT (Ion Torrent Proton) run: 1.4M spots, 116.2M bases, 89Mb downloads

Submitted by: NCBI (GEO)

Study: DSB-induced transcription of pLac-Tet plasmid in cell-free extracts.

PRJNA304447 • SRP066810 • All experiments • All runs

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Raw reads of RNA products generated upon incubation of circular and linear pLac-Tet plasmid in human cell-free extract. Overall design: RNA profiles of products generated upon incubation of circular and linear pLac-Tet plasmid in human cell-free extract obtained by deep sequencing using Ion Proton. pLac-Tet plasmid sequence prepared by cloning a fragment of 556 bp which contains an I-SceI site flanked by 3 Tet and 8 Lac repetitive elements into SfiI site of pMK-RQ vector (GENEART).

Sample: Linear plasmid

SAMN04302165 • SRS1183857 • All experiments • All runs

Organism: Synthetic plasmid

Library:

Instrument: Ion Torrent Proton

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Reaction RNA products were purified by phenol-chloroform extraction and precipitated overnight at -20°C in ethanol. Reaction RNA products were treated with DNase I and size-selected by gel. Ion Total RNA-Seq Kit v2 for strand-specific RNA sequencing. No shearing step was performed in order to preserve the original length of the molecules.

Experiment attributes:

GEO Accession: GSM1958347

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 1.4M spots, 116.2M bases, 89Mb

Run	# of Spots	# of Bases	Size	Published
SRR2966824	1,399,223	116.2M	89Mb	2017-12-17

ID:: 2058355

SRA

Sequence Read Archive

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